About Test

About SIBO Lactulose Test

The hydrogen and methane breath collection guidelines below are to be followed for all of our hydrogen and methane breath tests.

• No smoking, (including second-hand smoke) for at least one (1) hour before or at any time during the breath collection.
• No sleeping or vigorous exercise for at least one (1) hour before or at any time during the breath collection.
• If you recently had antibiotic therapy, colonoscopy, barium studies or enemas, please wait 14 days (or as directed by your clinician) after completion of the above mentioned prior to starting your breath test.
• Require a 24 hour diet preparation. The first 12 hours prior to your breath collection, you must limit your diet. Foods that you CAN eat during the first 12 hours of your preparation include: Baked or broiled chicken, fish (salt and pepper only), white bread (only), plain steamed white rice, eggs, clear chicken, beef or bone broth (no vegetables pieces), black coffee and tea. The second 12 hours prior to collecting your breath you will fast. Do not eat or drink anything other than water.

Preparation for the Test

Review the additional enclosed Preparation Guidelines prior to performing this test!

Any recent antibiotic treatment, barium study, colonoscopy, runny diarrhea or similar conditions can affect the test; therefore, DO NOT perform this test within 14 days of any of these conditions.

• Avoid foods listed in the Preparation Guidelines sheet 12 hours prior to fasting.
• No smoking, including second-hand smoke, for at least 1 hour before or at any time during the test.
• No sleeping or vigorous exercise for at least 1 hour before or at any time during the test.
• With the exception of water, do not eat/drink anything while fasting or during the test! This can cause false-positive readings or cause your test to be unreadable.
• Suggested meals prior to fasting are located in the Preparation Guidelines sheet.
• If you are on any medication or special diets that conflict with these test instructions, it is recommended to speak with your physician before performing this test.

Prepare Test Solution - DO NOT DRINK YET!!!

1. Mix the lactulose into 8-10 ounces of water.
2. Set drink aside, you will consume the solution after you collect your baseline sample.

Performing the Test (Collection Steps)

Collect your baseline sample following steps 1-4.

	Hold the EasySampler device in one hand and a collection tube in the other hand. You will only exhale once per each sample collection. Take a normal (not deep) breath in; close your mouth around the mouthpiece then blow out normally.
	Exhale once per each sample collection. As you exhale the bag fills with air. Keep it inflated. (There is a small hole in the bag, this is intentional). During your exhalation, insert the test tube into the needle holder completely so the stopper on the tube is punctured.
	Remove the test tube after 1-2 seconds. Keep the bag inflated until after the test tube is removed from the test tube holder.
	Complete the tube label provided. Make certain you label the Sample # correctly or your results will be inconclusive.

Drink the entire test solution you prepared and then wait 20 minutes.

Collect one breath sample every 20 minutes until all test tubes are used.

Collect all samples following the Sample Chart and Collection Steps 1-4.

Put collection test tubes in the bubble bag(s). Place the bubble bag(s), any paperwork, and the EasySampler device back in the cardboard container, and return to the laboratory for analysis immediately.

Return the kit immediately for analysis.

Your breath sample is only stable for 14 days after collection.